The content of platelet coaggregates with αβ-, γδ-Т-lymphocytes and their some minor subpopulations in the blood of healthy children

• Yelena N. Bogomyagkova
• Aleksey V. Solpov
• Yuriy A. Vitkovskiy
• Pavel P. Tereshkov

Abstract

Introduction. Platelets are able to enter into contact adhesive interactions with almost all blood cells. The formation of coaggregates of platelets and leukocytes in the bloodstream plays a certain role in the physiological processes of cell migration, and the degree of their interaction changes in pathology. In the literature, information on the adhesive interaction of platelets with leukocytes, and especially with lymphocytes and their subpopulations in the blood of healthy children is scarce.

The aim of the study – to research the relative and absolute content of coaggregates of platelets with lymphocytes, T-lymphocytes, αβ- and γδ-T-lymphocytes, their minor subpopulations – double negative and double positive T-lymphocytes (DNT and DPT cells) in the peripheral blood of healthy children of different age, as well as an assessment of the correlation between the studied parameters and the age of children.

Material and methods. The objects of the study were venous blood samples from 83 healthy children (boys and girls) aged from 7 months to 14 years. Monoclonal antibodies conjugated with various fluorochromes were used to detect lymphocyte-platelet coaggregates. Determination of the studied parameters was carried out using flow cytometry.

Results. The relative content and absolute amount of lymphocyte-platelet coaggregates in healthy children of different age groups have been investigated. A decrease in the absolute number of platelet coaggregates with T-lymphocytes, namely, with αβ-T-lymphocytes in the blood of older children, was established. At the same time, there was an increase in the relative content of the total pool of leukocyte-platelet coaggregates and coaggregates (platelets) with double negative T cells in older children compared with younger children.

Conclusion. The content of lymphocyte-platelet coaggregates depends on age. The absolute number of coaggregates of platelets with T-lymphocytes, αβ-T-lymphocytes decreases with age, and the content of coaggregates with γδ-T-lymphocytes remains unchanged. At the same time, there was an increase in the relative content of the total pool of leukocyte-platelet coaggregates and coaggregates (platelets) with double negative T cells in older children.

Keywords:lymphocyte-platelet adhesion; lymphocyte-platelet coaggregates

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