Characteristics of the cytokine network fragment (TNF–IL-1–IL-4–IL-6–IL-8–IL-10–VEGF) in the peripheral blood of healthy women and patients with leiomyoma
AbstractIntroduction. The axiom of immunology is the opinion that numerous cytokines, chemokines and growth factors act within the single cytokine network, which suggests the relevance of their research not in an isolated form, but in the form of interacting elements – pleiades.
Aim – to evaluate changes in the interdependent of the TNF–IL-1–IL-4–IL-6–IL-8–IL-10–VEGF cytokine group serum levels in healthy women and patients with uterine leiomyoma.
Material and methods. Using the observational version «case–control» study, 109 women 23 to 61 years old with leiomyoma (LM) and 92 women of the same age without this pathology were examined. All women underwent general clinical, standard instrumental and laboratory examinations. Cytokines levels were determined by the ELISA using «Vector Best» test systems (Russia). To detect the relationship between the studied markers, the correlation analysis was performed using the Spearman correlation coefficient, followed by graphical visualization and analysis of the structure of correlation relationships with the correlation graph method by Cytoscape 3.10.2.
Results. It was found that the development of uterine leiomyoma was accompanied by a significant (in fold) up-regulation of TNF, IL-1, IL-4, IL-6, IL-8 serum levels, in combination with the VEGF serum level down-regulation. Three variants of correlation pleiades were obtained – a group of general (nonspecific) correlations and two groups of orthogonal correlograms – one of them is characteristic only for the comparison group, and the second group is specific only for patients with LM.
Conclusion. The benign tumor process of the myometrium significantly affects the nature of functional connections in the studied fragment of the general cytokine network, which is clearly illustrated by changes in graphic elements in the correlation graph with the allocation of two alternative orthogonal groups of correlation galaxy, which are detected in healthy women and in patients with uterine leiomyoma.
Keywords:cytokine network; TNF; IL-1; IL-4; IL-6; IL-8; IL-10; VEGF; ELISA; correlation graphs; uterine leiomyoma
For citation: Konenkov V.I., Prokofiev V.F., Shevchenko A.V., Koroleva E.G., Timofeeva Yu.S., Aidagulova S.V., Marinkin I.O. Characteristics of the cytokine network fragment (TNF–IL-1–IL-4–IL-6–IL-8–IL-10–VEGF) in the peripheral blood of healthy women and patients with leiomyoma. Immunologiya. 2024; 45 (3): 312–20. DOI: https://doi.org/10.33029/1816-2134-2024-45-3-312-320 (in Russian)
Funding. The work was carried out within the framework of the State Assignment of RICEL – Branch of the FRC IC&G SB RAS of the MSHE of Russia for basic scientific research – FWNR-2022-0009 (state registration number R&D 1021060908897-3). Open publication of the research results is allowed.
Conflict of interests. The authors declare no conflict of interests.
Authors’ contribution. The concept and design of the study – Konenkov V.I., Marinkin I.O.; collection and processing of primary material – Koroleva E.G., Timofeeva Yu.S.; analysis of literature data – Timofeeva Yu.S., Aidagulova S.V.; conducting enzyme-linked immunosorbent assay – Shevchenko A.V.; statistical data processing – Prokofiev V.F.; writing and editing text – Konenkov V.I., Prokofiev V.F., Aidagulova S.V.
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